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DOI : 10.17640/KSWST.2016.24.4.35 ,    Vol.24, No.4, 35 ~ 47, 2016
Study on Water Purification Performance Evaluation of Microorganisms Isolated from Jeju Island
김우석 Woo Suk Kim , 박준석 Jun Seok Park , 서대석 Dae Seuk Seo , 김봉균 Bong Kyun Kim , 손승욱 Seung Wook Son , 박재로 Jae Roh Park
In this study, we collected samples from rivers in Jeju Island and other places where the self-purification capacity of rivers had been recovered by utilizing effective microorganisms(EM). And then after separating microorganisms from among the samples, we evaluated each microorganism’s water purification. In addition, we performed DNA sequencing by means of the 16S rRNA gene (16S rDNA) sequencing method for the identification of microorganisms capable of improving water quality and sought to register gene sequence information in GenBank at the National Center for Biotechnology Information (NCBI). According to the COD experiment results, C1, B4 and C6 showed the average removal efficiency of 21.3, 23.4 and 38.6% respectively. According to the T-N experiment results, the respective strains of B4, B6, M3 and C1 showed the average removal efficiency of 26.8, 34.9, 28.3 and 23.3% respectively. According to the T-P experiment results, the respective strains of B4, B6, C1 and C4 showed the average removal efficiency of 15.0, 19.4, 15.1 and 9.1% respectively. By examining the present study, it is judged that the strain of B6 has the best water purification performance. As a result of its identification, the strain of B6 showed 99.66% similarity to the strain of Bacillus subtilis. Moreover, the gene sequence information was registered in GenBank at NCBI in the USA, thereby being assigned the accession number of ‘KU672511’.
Key Words
Jeju Island, Water Purification, Identification, Bacillus subtilis
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